United Kingdom (UK)
Recombinant monoclonal antibody to Fya. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma Hima-19.
UniProt Accession Number of Target Protein: Q5Y7A2
Alternative Name(s) of Target: Duffy antigen; Human immunochemistry monoclonal antibody-19; Fy(a); Duffy antigen Fya; FY; hima19; hima 19
Immunogen: This human-murine chimeric antibody was derived from the anti-Fya murine monoclonal antibody MIMA-19 (Ab01349); cDNA encoding the VH and VL gene segments from MIMA-19-secreting hybridoma cells were inserted into human IgM expression vectors.
Specificity: This antibody is specific for the Fya Duffy blood group antigen.
Application Notes: This antibody has been used to successfully determine the phenotype of DAT-positive red blood cell samples, which are otherwise challenging to phenotype due to the presence of surface-bound autoantibodies and/or complement (Lee et al, 2004).
Antibody first published in:
Lee et al. Applications of murine and humanized chimeric monoclonal antibodies for red cell phenotyping. Br J Haematol. 2004 Jul;126(2):277-81. PMID:15238151
Note on publication:
Describes the original generation of this antibody.