United Kingdom (UK)
Recombinant monoclonal antibody to GFAP. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 10G1G11H7.
UniProt Accession Number of Target Protein: P14136
Alternative Name(s) of Target: Glial fibrillary acidic protein; CGMCCNO.17407; 6F2
Immunogen: The original antibody was generated by immunizing mice with purified recombinant GFAP protein.
Specificity: The original antibody recognizes the recombinant protein GFAP. GFAP is a class-III intermediate filament. It is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Application Notes: The antibody specifically detected GFAP protein by Western blot analysis. This antibody and the commercial antibody 6F2 were used for immunohistochemistry on astrocytoma, glioma, pituitary and brain tissue and the results were compared. The antibody 10G1G11H7 had accurate staining location on the cell membrane, the staining was clear without non-specific staining, and the background was clean. Further, the results were consistent with the ones of the commercially available antibody GFAP (6F2), indicating that the specificity of the antibodies is equivalent (CN110903389A).
Antibody first published in: PMID:
Western blot using anti-GFAP antibody 10G1G11H7 (Ab03302). U251 (A) cells lysate, human brain cerebellum (B), and cerebral cortex (C) tissue lysates (35µg protein in RIPA buffer) were resolved on an SDS-PAGE gel, and blots were probed with the chimeric rabbit version of 10G1G11H7 (Ab03302-23.0) at 0.001µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and protein was detected by chemiluminescence.
Immunofluorescence staining of U251 cells with anti-GFAP antibody 10G1G11H7 (Ab03302). Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton, on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 10G1G11H7 (Ab03302-23.0) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, Ab03302-23.0, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.
Flow cytometry using anti-GFAP antibody 10G1G11H7 (Ab03302). Paraformaldehyde fixed U251 cells permeabilized with 0.5% Triton were stained with the anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of 10G1G11H7 (Ab03302-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000, and the cells were analyzed using a FACSCanto flow-cytometer.