United Kingdom (UK)
Recombinant monoclonal antibody to GP25-200. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 4H9.
Alternative Name(s) of Target: GP25-200 antigen
Immunogen: The original antibody was generated by immunizing female BALB/c mice intraperitoneally and subcutaneously with purified P23 emulsified in monophosphoryl A-trehalose dimycolate adjuvant.
Specificity: This antibody binds the GP25-200 antigen of Cryptosporidium parvum. Cryptosporidium parvum is a coccidian parasite that infects intestinal epithelium and causes diarrheal disease in humans and other agriculturally important food animals for which specific therapy and immunoprophylaxis are unavailable. Cryptosporidiosis is self-limiting in hosts with normal immune systems; however, in neonates, the elderly, and hosts having congenital or acquired immunodeficiency diseases or chemotherapy-induced immunosuppression, cryptosporidial enterocolitis may become chronic and have severe consequences.
Application Notes: This antibody can be used for the identification of sporozoites and GP25-200 antigen of C. parvum using western blot. The identification of sporozoite-neutralizing antibody was done using ELISA (PMID: 10768951). This antibody in combination with PLA2 or LL37 was also used in the generation of a biocide fusion proteins to kill C. parvum sporozoites in vitro and in vivo (PMID: 20086143).
Antibody first published in:
Schaefer et al. Characterization and formulation of multiple epitope-specific neutralizing monoclonal antibodies for passive immunization against cryptosporidiosis. Infect Immun. 2000 May;68(5):2608-16. PMID:10768951
Note on publication:
Describes the generation and characterization of antibodies against multiple epitope of C. parvum to provide passive immunization against Cryptosporidiosis.