United Kingdom (UK)
Recombinant monoclonal antibody to GXM. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the human B cell clone 2E9.
Alternative Name(s) of Target: Glucuronoxylomannan; Cryptococcus neoformans capsular polysaccharide; CNPS
Immunogen: The original antibody was generated from the peripheral blood lymphocytes of glucuronoxylomannan-tetanus toxoid conjugate (GXM-TT)-immunized individual.
Specificity: This antibody is specific for GXM or glucuronoxylomannan from Cryptococcus neoformans. GXM is a polysaccharide expressed on the surface of C. neoformans. This antibody binds an epitope of C. neoformans that is different from the epitope of another anti-GXM antibody 2H1.
Application Notes: This antibody was used for the immunofluorescent labelling of C. neoformans serotype D and A. This antibody can be used in combination with another anti-GXM antibody 2H1 for detection of all four serotypes of C. neoformans GXM using sandwich ELISA. The antibody 2H1 is used as capture antibody and 2E9 is used as a detection antibody (PMID: 7622223).
Antibody first published in:
Pirofski et al. Analysis of human monoclonal antibodies elicited by vaccination with a Cryptococcus neoformans glucuronoxylomannan capsular polysaccharide vaccine. Infect Immun. 1995 Aug;63(8):3005-14. PMID:7622223
Note on publication:
Describes the generation of two human antibodies from the peripheral blood lymphocytes (PBLs) of a GXMTT-immunized individual.