United Kingdom (UK)
Recombinant monoclonal antibody to Gelsolin. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the llama peripheral lymphocytes GsnVHH 13.
UniProt Accession Number of Target Protein: P06396
Alternative Name(s) of Target: GSN; AGEL; ADF; Actin-depolymerizing factor; Brevin
Immunogen: The original antibody was generated by immunizing a llama subcutaneously with human recombinant gelsolin.
Specificity: This antibody recognizes Ca2+ activated gelsolin. This antibody specifically recognizies and epitope in the G4-G5 domain of gelsolin.
Application Notes: Gelsolin is a multifunctional actin-binding protein that controls cellular actin organization and migration. The original VHH version of this antibody binds calcium activated gelsolin with a Kd of ~10 nM. The specificity of the antibody towards gelsolin was confirmed with ELISA. GsnVHH 13 expressed as intrabodies immunoprecipitate endogenous gelsolin. His-tagged GsnVHH 13 can be used to identify gelsolin in a western blot assay. This antibody can trace gelsolin in membrane ruffles of EGF-stimulated MCF-7 cells and delay cellmigration without affecting F-actin severing/capping oractin nucleation activities by gelsolin as demonstrated by immunofluorescense staining. (PMID:20140750).
Antibody first published in:
Abbeele et al. A llama-derived gelsolin single-domain antibody blocks gelsolin-G-actin interaction. Cell Mol Life Sci. 2010 May;67(9):1519-35. PMID:20140750
Note on publication:
Describes the generation, characterization, epitope mapping and crystal structure of this antibody.