United Kingdom (UK)
Recombinant monoclonal antibody to Genome polyprotein. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 1H10.
UniProt Accession Number of Target Protein: A9LID6
Alternative Name(s) of Target: pol; Dengue virus type 3
Immunogen: The original antibody was raised from PMBCs from humans previously infected with dengue fever.
Specificity: This antibody is specific for the precursor membrane of dengue fever. L3 and S5 are important amino acids for its epitope.
Application Notes: The original antibody was used for an ELISA on whole dengue virus (Smith et al., 2015; PMID:26512092), and on DENV3 (Wirawan et al., 2019; PMID:30471923). This antibody showed binding to all dengue virus types. This antibody was used for western blotting on PrM protein. This antibody was used for an ADE assay. This assay showed that this antibody can enhance a dengue fever infection (Smith et al., 2015; PMID:26512092, Wirawan et al., 2019; PMID:30471923). The structure of this antibody was determined using cryo-EM. This antibody was used for a virus-liposome co-floatation assay on LoVo cells with DENV2. This antibody was used for BioLayer Interferometry analysis on recombinant prM:E in PBS-T. This antibody was also used for Hydrogen Deuterium Exchange on the prM:E protein (Wirawan et al., 2019; PMID:30471923).
Antibody first published in:
Smith et al. Dengue Virus prM-Specific Human Monoclonal Antibodies with Virus Replication-Enhancing Properties Recognize a Single Immunodominant Antigenic Site J Virol. 2015 Oct 28;90(2):780-9. PMID:26512092
Note on publication:
The original paper reports the isolation of a large panel of naturally occurring human monoclonal antibodies (MAbs) obtained from subjects following primary DENV serotype 1, 2, or 3 or secondary natural DENV infections or following primary DENV serotype 1 live attenuated virus vaccination to determine the antigenic landscape on the prM protein that is recognized by human antibodies.