United Kingdom (UK)
Recombinant monoclonal antibody to Giantin. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody TA10.
Alternative Name(s) of Target: Golgin subfamily B member 1; 372 kDa Golgi complex-associated protein; GCP372; Macrogolgin
Immunogen: The original antibody was generated via phage display from the Griffin.1 library by screening against Rat liver Golgi (RLG) complexes derived from rat liver.
Specificity: This antibody is specific for a 79 amino acid long region between amino acids 1462 to 1540 of the full human sequence (corresponding to amino acids 1399 to 1478 in the rat homologue) in Giantin, a Golgi matrix protein.
Application Notes: The original format of this antibody (scFv) was successfully used in a Western blot and for in vitro immunofluorescence straining of Giantin in HeLa cells. siRNA knock-down of Giantin reduced TA10 staining, confirming its specificity. Furthermore, an in vivo expressed TA10-GFP construct successfully stained HeLa cells (Nizak et al., 2003; PMID: 14617357). The scFv-Fc fusion version of this antibody — hTA10 — detected Giantin when cells were fixed by paraformaldehyde (Moutel et al., 2009; PMID: 19245715). Precise characterization of the TA10 target was achieved; a region of 79 amino acids, representing less than 2.5% of the entire protein, was determined as its binding site (Vielemeyer et al., 2010; PMID: 20727208).
Antibody first published in:
Nizak et al. Recombinant antibodies against subcellular fractions used to track endogenous Golgi protein dynamics in vivo Traffic. 2003 Nov;4(11):739-53. doi: 10.1034/j.1600-0854.2003.00132.x PMID:14617357
Note on publication:
The original publication demonstrates the use of recombinant antibodies selected through phage display against subcellular fractions.