United Kingdom (UK)
Recombinant monoclonal antibody to Glypican 3. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma AB13E3.
UniProt Accession Number of Target Protein: P51654
Alternative Name(s) of Target: GPC3; Glypican-3; GPC-3; GTR2-2; MXR7; OCI5; DGSX; Intestinal protein OCI-5; GPC3#4; DWX-1
Immunogen: The parental mouse antibody GPC3#4 was generated by immunizing BALB/c mice with 50μg of purified human GPC-3 antigen emulsified in Freund's complete adjuvant. This original antibody was generated be grafting CDRs of the parental mouse antibody onto human framework region.
Specificity: This antibody binds human glypican 3 (GPC3).Glypican-3 is an oncofetal antigen that belongs to the glypican family of glycosyl-phosphatidylinositol-anchored heparin sulfate proteoglycans. It negatively regulates the hedgehog signaling pathway and positively regulates the canonical Wnt signaling pathway. Multiple studies have revealed that GPC-3 is overexpressed in hepatocellular carcinoma (HCC).
Application Notes: The binding specificity of this antibody for human glypican 3 was determined using ELISA. The original IgG1 version of this antibody binds human GPC3 with an affinity of Kd = 2.84 pM. This antibody can bind GPC3 expressed on the surface of PLC/PRF/5 and Huh7 cells in an in vitro binding assay. This antibody also positively shows ADCC activity on PLC/PRF/5 cells expressing glypican 3 (CN109988240).
Antibody first published in: PMID: