United Kingdom (UK)
Recombinant monoclonal antibody to HA tag. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 12CA5.
UniProt Accession Number of Target Protein: Q3TBD2
Alternative Name(s) of Target: Rho GTPase-activating protein 45; Arhgap45; Minor histocompatibility protein HA-1
Immunogen: This antibody was raised by immunizing balb/c mice with a 9 amino acid petide to influenza virus hemagglutinin.
Specificity: This antibody is specific for the HA-tag. HA contains a GTPase activator for the Rho-type GTPases (RhoGAP) domain that would be able to negatively regulate the actin cytoskeleton as well as cell spreading. However, also contains N-terminally a BAR-domin which is able to play an autoinhibitory effect on this RhoGAP activity.
Application Notes: The mouse version of this antibody has shown the ability to immunoprecipitate HA bound to BAX from cell lysates (Cheng et al, 1996; pmid:8596636). HA tagged SIM1 was immoprecipitated using the mouse version of this antibody. Other proteins present that lacked the HA tag were not immunoprecipitated (Michaud et al, 2000; pmid:10640708). A western blot has detected HA bound to PI4P5K using the mouse version of this antibody (Shibasaki et al, 1997; pmid:9065410). The HOXC6 protein bound to a HA-tag was immunoprecipitated using the mouse version of this antibody (McCabe et al, 2008; pmid:18339881). Cells expressing MLS2 fused to the HA epitope were stained using the mouse version of this antibody. Further the MSL2-HA protein was immuoprecipitated using this antibody (Smith et al, 2000; pmid:10594033). The Pat1 gene tagged with HA can be immunoprecipitated from lysed yeast cells using the mouse version of this antibody. Furthermore, the mouse version of this antibody can detect pat1-HA in a western blot. The western blot was preformed to determine the effect of topoisomerase II activity on chromosome segregation (Wang et al, 1996; pmid:8972867). HA tagged proteins can be stained using the mouse version of this antibody. Furthermore, the SPOP protein bound to a HA tag is able to be detected by the mouse version of this antibody on a western blot. The western blot was preformed to identify a novel antigen found in serum from a sclerodoma patient (Nagai et al, 1997; pmid:9414087).
Antibody first published in:
Cheng et al. Bax-independent inhibition of apoptosis by Bcl-XL Nature. 1996 Feb 8;379(6565):554-6. PMID:8596636
Note on publication:
Using site-specific mutagenesis,it is shown here that the amino-acid residues critical for protection of cells by Bcl-XL against Sindbis virus-induced apoptosis are clustered within the Bcl-2-homology regions 1 and 2 (BH1 and BH2 regions).