United Kingdom (UK)
Recombinant monoclonal antibody to HCV core protein. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 19D9D6.
Alternative Name(s) of Target: Hepatitis C virus core protein
Immunogen: The original antibody was generated by immunizing BALB/c JYco mice with a truncated recombinant protein corresponding to the immunodominant region (residues 1–120) of the Hepatitis C Virus (HCV) purified nucleocapsid protein (HCV C120-GST fusion protein).
Specificity: This antibody is specific for the protein sequence QIVGGVYLL located at residues 29-37 of the HCV core protein. Hepatitis C virus (HCV) is the primary cause of hepatitis transmitted through blood transfusion. It is an enveloped virus that contains a single-stranded, positive-sense RNA genome of approximately 9600 nucleosides. This genome encodes a polyprotein of 3011 amino acids, which undergoes post-translational cleavage to form both structural and nonstructural proteins. Among the structural proteins, the core protein is derived from the amino terminus of the viral polypeptide (amino acids 1-191).
Application Notes: This antibody was used for an indirect ELISA to detect anti-HCV core response in human sera, an ELISA competition assays to assess the interaction between monoclonal antibodies and anticore human sera, a sandwich ELISA for the capture and detection of viral core antigen in chronic HCV patient sera, and a sandwich ELISA for the detection of recombinant core protein (Jolivet-Reynaud et al., 1998; PMID: 9829633). The crystal structure of the Fab version of this antibody was solved in complex with an HCV core protein-derived peptide (residues 13-40), revealing the hydrophobic nature of the recognized epitope (Ménez et al., 2003; PMID: 12574359).
Antibody first published in:
Jolivet-Reynaud et al. HCV core immunodominant region analysis using mouse monoclonal antibodies and human sera: characterization of major epitopes useful for antigen detection J Med Virol. 1998 Dec;56(4):300-9. doi: 10.1002/(sici)1096-9071(199812)56:43.0.co;2-8 PMID:9829633
Note on publication:
The original publication focuses on the generation of monoclonal antibodies (MAbs) against the immunodominant region of the hepatitis C virus (HCV) core protein and the characterization of major epitopes within this region that are useful for antigen detection.