United Kingdom (UK)
Recombinant monoclonal antibody to HSP65. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody G8.
UniProt Accession Number of Target Protein: P0A521
Alternative Name(s) of Target: Chaperonin-60 2; Heat shock protein 65; Chaperonin GroEL 2; 60 kDa chaperonin 2; 65 kDa antigen; Antigen A; Cell wall protein A
Immunogen: This antibody was generated by panning a phage displayed repertoire derived from the immunoglobulin genes of the chicken against HSP65.
Specificity: This antibody is specific for the HSP65 protein from Mycobacterium bovis. Together with its co-chaperonin GroES, HSP65 plays an essential role in assisting protein folding. Mycobacterium bovis is the causative agent of tuberculosis in cattle.
Application Notes: An ELISA was performed using the scFv version of this antibody on HSP65 to determine how efficiently the antibody can bind the antigen. Furthermore, an immunoblot was performed on HSP65 using this antibody (Wemmer et al, 2010; pmid:20299243).
Antibody first published in:
Wemmer et al. Chicken scFvs and bivalent scFv-C(H) fusions directed against HSP65 of Mycobacterium bovis. Biologicals. 2010 May;38(3):407-14. PMID:20299243
Note on publication:
Two chicken single-chain variable region antibody fragments (scFvs) that recognised the 65 kDa heat-shock protein (HSP65) of Mycobacterium bovis were selected from a large semi-synthetic phage displayed library. Both recognised HSP65 in indirect enzyme-linked immunosorbent assay (ELISA) and immunoblots and retained their activity during storage.