United Kingdom (UK)
Recombinant monoclonal antibody to Human A-type (A1; A2) erythrocytes. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma W6/1.
Immunogen: W6/1 was prepared by immunizing BALB/c mice with membrane from human tonsil cells (present as a result of contaminating erythrocyte membrane in the tonsil leucocyte preparation).
Specificity: W6/1 binds to A1 and A2 erythrocytes and does not bind to B or O erythrocytes (detects a polymorphic determinant of erythrocytes correlating with blood group A). When saturating amounts of secondary antibody are used, binding of W6/1 is 5 times as great on type A1 than A2.
Application Notes: W6/1 antibody can be used as an erythrocyte cell type marker and can cause hemogglutination of A-type erythrocytes.
Antibody first published in:
Barnstable et al Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysis. Cell 14:9-20 1978 PMID:667938
Note on publication:
Describes the production of W6/1 antibody and determination of its binding specificity.