United Kingdom (UK)
Recombinant monoclonal antibody to IFN-y. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybrodoma YC1.
UniProt Accession Number of Target Protein: P49708
Alternative Name(s) of Target: Interferon gamma; Interferon-gamma; IFN-gamma; IFNG; IFN-g; Type II interferon
Immunogen: The original antibody was raised by immunizing BALB/c mice with recombinant chicken IFN-y.
Specificity: The antibody binds specifically to IFN-gamma, a dimerized soluble cytokine and the only member of the type II class of interferon.
Application Notes: The specificity of this antibody (IgG2b) was confirmed by ELISA analysis. The IFN-g ELISA was also capable of measuring cytokine levels in the sera of chickens orally infected with Eimeria maxima. The antibody detected the recombinant IFN-y by western blot analysis. The antibody was capable of neutralizing IFN-g bioactivity against virus-infected cells (Yun et al., 2000; pmid: 10713342).
Antibody first published in:
Yun et al. Chicken IFN-gamma monoclonal antibodies and their application in enzyme‐linked immunosorbent assay. Vet I mmunol Immunopathol. 2000;73(3‐4):297‐308. PMID:10713342
Note on publication:
The paper describes the generation and characterization of the antibody, specific for chicken IFN-y for the purpose of developing an IFN-y-specific enzyme-linked immunosorbent assay.