United Kingdom (UK)
Recombinant monoclonal antibody to IL-15. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybrodoma M4-7.
UniProt Accession Number of Target Protein: F1NZN5
Alternative Name(s) of Target: Interleukin-15; Interleukin; IL15
Immunogen: The original antibody was raised by immunising BALB/c mice with IL-15.
Specificity: The antibody binds specifically to chicken IL-15, a cytokine with structural similarity to interleukin-2, another T cell growth factor important in the regulation of cell-mediated immunity. The antibody did not react with IL-2 or IFN-y.
Application Notes: The specificity of this antibody (IgG2b) was confirmed in ELISA analysis. The antibody detected the recombinant chIL-15 by western blot analysis. Further, the antibody inhibited the IL-15-induced proliferation of splenic lymphoblast cells in a dose-dependent manner, when used at 20 mg/ml, the antibody inhibited proliferation by 56.7% (Min et al, 2002; pmid: 12088644).
Antibody first published in:
Min et al. Development and characterization of monoclonal antibodies to chicken interleukin‐15. Vet Im munol Immunopathol. 2002;88(1‐2):49‐56. PMID:12088644
Note on publication:
The paper describes the generation and characterization of the antibody, which is specific for chIL-15.