United Kingdom (UK)
Recombinant monoclonal antibody to IL-17A. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma MM17F3.
UniProt Accession Number of Target Protein: Q62386
Alternative Name(s) of Target: Interleukin-17A; IL17A; Cytotoxic T-lymphocyte-associated antigen 8; CTLA-8
Immunogen: This antibody was raised by immunising C57Bl/6 mice with IL-17A cross-linked to ovalbumin to generate a highly immunogenic complex.
Specificity: This antibody is highly specific for murine IL-17A; in ELISA analysis, no cross-reactivity with IL‐17B, IL‐17C, IL‐17D, IL‐17E or IL‐17F was observed.
Application Notes: The specificity of this antibody has been confirmed in ELISA analysis (Uyttenhove et al, 2006). This antibody inhibits IL‐17A‐induced IL‐6 production by a Cl1D fibroblastic cell line in vitro (Uyttenhove et al, 2006). This antibody has been shown to prevent experimental autoimmune encephalomyelitis (EAE) disease development in vivo (Uyttenhove et al, 2006), as well as protecting C57Bl/6 mice from myelin oligdendrocyte glycoprotein (MOG)-induced EAE (Uyttenhove et al, 2007). Additionally, administration of this antibody inhibits collagen-induced arthritis (CIA) in IFN-γ receptor knockout mice (Kelchtermans et al, 2009).
Antibody first published in:
Uyttenhove et al. Development of an anti‐IL‐17A auto‐vaccine that prevents experimental auto‐immune encephalomyelitis Eur J Immunol. 2006 Nov;36(11):2868-74 PMID:17048276
Note on publication:
Describes the original generation of this antibody, and the characterisation of its IL-17A-inhibitory activity.
Flow-cytometry using the Anti-IL-17A antibody MM17F3 (Ab01266). Mouse splenocytes were stimulated with PMA and Ionomycin in presence of Monensin for 4 hours. The cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of MM17F3 (Ab01266-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.