United Kingdom (UK)
Recombinant monoclonal antibody to IL-18. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybrodoma E3.
UniProt Accession Number of Target Protein: Q9I8D2
Alternative Name(s) of Target: IGIF; Interleukin 18; interferon-y inducing factor; chIL18; Interleukin-18; IL 18; Chicken IL-18
Immunogen: The original antibody was raised by immunizing BALB/c mice with recombinant chicken IL-18.
Specificity: The antibody binds specifically to chicken IL-18, a cytokine that is produced by macrophages and other cells, and shows synergistic action with IL-12 to induce strong cell-mediated immunity following infection with microbial products, such as lipopolysaccharide.
Application Notes: The specificity of this antibody (IgG2a) was confirmed by ELISA analysis. The antibody detected the recombinant chIL-18 by western blot analysis (Hong et al, 2010; pmid: 20674988).
Antibody first published in:
Hong et al. Development and characterization of mouse monoclonal antibodies specific for chicken interleukin 18. Vet Immunol Immunopathol. 2010;138(1‐2):144‐148. PMID:20674988
Note on publication:
The paper describes the generation of the antibody, which is capable of detecting chIL18, and can be used for basic and applied research in poultry.