United Kingdom (UK)
Recombinant monoclonal antibody to IL-1A. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 9H10.
UniProt Accession Number of Target Protein: P01582
Alternative Name(s) of Target: IL1A; IL-1α; Interleukin-1 alpha; IL-1 alpha
Immunogen: The original antibody was generated by immunizing IL-1α/β double KO mice with mouse IL-1 alpha.
Specificity: This antibody binds mouse Interleukin-1 alpha, which is a cytokine constitutively present intracellularly in nearly all resting non-hematopoietic cells that plays an important role in inflammation and bridges the innate and adaptive immune systems. IL-1α is produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells.
Application Notes: The original mouse IgG antibody binds mouse IL-1A with a binding affinity of Kd= 0.17 nM. The binding characterization of this antibody was done using ELISA. In vitro antibody potency was determined by its ability to inhibit IL-8 production by MRC-5 cells. This antibody was able to inhibit IL-8 production by MRC-5 cells with an IC50 of 2.00E−10M. This antibody was used in combination with 10G11 (anti- mouse IL-1B) antibody for the generation of anti-mouse IL-1α/β DVD-Ig molecule (US7612181).
Antibody first published in: PMID: