United Kingdom (UK)
Recombinant monoclonal antibody to IL-27p28. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma MM27-7B1.
UniProt Accession Number of Target Protein: Q8K3I6
Alternative Name(s) of Target: MM27.7B1; MM27 7B1; MM277B1; Interleukin-27 subunit alpha; IL-27 subunit alpha; IL-27-A; IL27-A; p28; Il27; Il27a
Immunogen: This antibody was raised by immunising mice with a p28–EBI3 fusion protein linked to ovalbumin polymers covered with glutaraldehyde (OVAglu).
Specificity: This antibody is specific for the IL-27 p28 subunit.
Application Notes: This antibody has been used to detect intracellular IL-27 in flow cytometric analysis (Bosmann et al, 2014), and to detect serum and cell culture supernatant IL-27 in sandwich ELISA analysis (Marillier et al, 2014). This antibody blocks IL-27, and is able to restore IL‐9 and IL‐17A production in the presence of IL‐27 (Uyttenhove et al, 2011). Administration of this antibody blocks graft-versus-host disease upon B6 cell transfer to B6D2 recipient mice (Marillier et al, 2014).
Antibody first published in:
Uyttenhove et al. Amine‐reactive OVA multimers for auto‐vaccination against cytokines and other mediators: perspectives illustrated for GCP‐2 in L. major infection J. Leukoc. Biol.2011. 89: 1001–1007 PMID:21385949
Note on publication:
Describes the original generation of this antibody, and the characterisation of its IL-27-inhibitory activity.