United Kingdom (UK)
Recombinant monoclonal antibody to IL-4. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 12-6-68.
UniProt Accession Number of Target Protein: P05112
Alternative Name(s) of Target: IL4; BSF-1; Interleukin-4; B-cell stimulatory factor 1; Binetrakin; Lymphocyte stimulatory factor 1; Pitrakinra
Immunogen: The original antibody was generated by immunizing BALB/c mice subcutaneously with 25 µg of human IL-4 in 100 µl of 150 mM sodium chloride emulsified with 100 µl of complete Freund’s adjuvant.
Specificity: This antibody binds human IL-4. Interleukin-4 is a cytokine secreted primarily by mast cells, T-cells, eosinophils, and basophils that plays a role in regulating antibody production, hematopoiesis and inflammation, and the development of effector T-cell responses. It induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. It also induces expression of class II MHC molecules on resting B-cells and enhances both secretion and cell surface expression of IgE and IgG1.
Application Notes: The binding of this antibody to human IL-4 was confirmed with ELISA (PMID: 12825686).
Antibody first published in:
Ehsani et al. Expression of anti human IL-4 and IL-6 scFvs in transgenic tobacco plants. Plant Mol Biol. 2003 May;52(1):17-29. PMID:12825686
Note on publication:
Describes the expression of two murine antibodies in Agrobacterium tumefaciens.