United Kingdom (UK)
Recombinant monoclonal antibody to IL-6. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the llama B-cell clone 61H7.
UniProt Accession Number of Target Protein: P05231
Alternative Name(s) of Target: IL6; IFNB2; BSF-2; CDF; Interleukin-6; IL-6; B-cell stimulatory factor 2; CTL differentiation factor; Hybridoma growth factor; Interferon beta-2; IFN-beta-2
Immunogen: The original antibody was generated by immunizations of Llama glama with recombinant IL-6 followed by construction of phage display library, selection, screeing and purification.
Specificity: This antibody recognizes and binds interleukin-6 on the top of the four-helix bundle near the N and C termini. Here the VL domain sterically interferes with the C-terminal domain of IL-6R (residues 195–295). The overlap of the 61H7 epitope and the site I surface concentrates around a cavity occupied by both the HCDR3 loop of 61H7 and the IL-6R molecule.
Application Notes: This antibody is recommended for identification of IL-6 in the sample. The ability of this antibody to compete with biotinylated IL-6 for binding to captured IL-6R was investigated using competitive ELISA. The in-vitro potency of the antibody was determined using a cell-based neutralizing bioassay using the B9 and 7TD1 cell line, which require IL-6 for survival and proliferation and respond to very low concentration of IL-6 (PMID: 27129274).
Antibody first published in:
Blanchetot et al. Structural Mimicry of Receptor Interaction by Antagonistic Interleukin-6 (IL-6) Antibodies. J Biol Chem. (2016); 291(26); 13846-54. PMID:27129274
Note on publication:
Describes the crystal structure and epitope characterization of the antibody.