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- Ab02026-10.0 Anti-IL-6 [68F2]
- Human
- Human IgG1
- Purified
- Ships in 4-5 weeks
- Ab02026-10.3 Anti-IL-6 [68F2]
- Human
- Human IgG1
- Fc Silent™
- Purified
- Ships in 4-5 weeks
- Ab02026-23.0 Anti-IL-6 [68F2]
- Human
- Rabbit IgG
- Purified
- In Stock
Recombinant monoclonal antibody to IL-6. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the llama B-cell clone 68F2.
UniProt Accession Number of Target Protein: P05231
Alternative Name(s) of Target: IL6; IFNB2; BSF-2; CDF; Interleukin-6; IL-6; B-cell stimulatory factor 2; CTL differentiation factor; Hybridoma growth factor; Interferon beta-2; IFN-beta-2
Immunogen: The original antibody was generated by immunizations of Llama glama with recombinant IL-6 followed by construction of phage display library, selection, screeing and purification.
Specificity: This antibody recognizes and binds interleukin-6 near the binding site I near the N-terminal part of α-helix 1 and the C-terminal part of α-helix 4 of the four-helix bundle, as well as the long loop between α-helices 1 and 2. This binding interface of 68F2 to a major extent overlaps with site I recognized by IL-6R.
Application Notes: This antibody is recommended for identification of IL-6 in the sample. The ability of this antibody to compete with biotinylated IL-6 for binding to captured IL-6R was investigated using competitive ELISA. The in-vitro potency of the antibody was determined using a cell-based neutralizing bioassay using the B9 and 7TD1 cell line, which require IL-6 for survival and proliferation and respond to very low concentration of IL-6 (PMID: 27129274).
Antibody first published in:
Blanchetot et al. Structural Mimicry of Receptor Interaction by Antagonistic Interleukin-6 (IL-6) Antibodies. J Biol Chem. (2016); 291(26); 13846-54. PMID:27129274
Note on publication:
Describes the crystal structure and epitope characterization of the antibody.
Western Blot using Anti-IL-6 antibody 68F2 (Ab02026). Human skeletal muscle tissue lysate (35µg protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 68F2 (Ab02026-23.0) at 0.3µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.