United Kingdom (UK)
Recombinant monoclonal antibody to IL-6R. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma D7715A7.
UniProt Accession Number of Target Protein: P22272
Alternative Name(s) of Target: CD126; 15A7; IL-6Ra; IL6R; Interleukin-6 receptor subunit alpha; Il6ra; IL-6 receptor subunit alpha; IL-6R subunit alpha; IL-6R-alpha; IL-6RA; IL-6R 1
Immunogen: This antibody was raised by immunising Lewis rats with OKT4 cells.
Specificity: This antibody is specific for murine IL-6R.
Application Notes: This antibody binds to IL-6R-positive cells, and blocks IL-6 binding (Vink et al, 1990; Coulie et al, 1990). This antibody is able to inhibit the growth of IL6-dependent plasmacytoma T1033C2 in vitro (Coulie et al, 1990). Administration of this antibody to mice prior to challenge with T1033C2 resulted in a protection rate of 60% and prolonged survival in tumour-bearing mice (Vink et al, 1990). This antibody has been used in flow cytometry to assess IL-6Ra expression (Hoge et al, 2013). When added to dendritic cells subject to CD40 activation in vitro, this antibody neutralises IL-6 and ablates the effect of CD40 activation (Grohmann et al, 2001).
Antibody first published in:
Coulie et al. A monoclonal antibody specific for the murine IL-6-receptor inhibits the growth of a mouse plasmacytoma in vivo. Curr Top Microbiol Immunol. 1990;166:43-6. PMID:2073815
Note on publication:
Describes the original generation of this antibody, and the characterisation of its ability to block IL-6 binding.
Immunofluorescence staining of mouse splenocytes using anti-IL-6R antibody (Ab01259) D7715A7. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix™ cover-slips and stained with the chimeric rabbit IgG version of D7715A7 (Ab01259-23.0) at 10 µg/ml followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab01259-23.0, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (Ab00102-23.0) followed by Alexa Fluor® 488 secondary antibody.
Flow-cytometry using the Anti-IL-6R antibody D7715A7 (Ab01259). Mouse splenocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, shaded line) or the rabbit IgG version of D7715A7 (Ab01259-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.