United Kingdom (UK)
Recombinant monoclonal antibody to Insulin. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma Ab 123 (mAb3, CC9C10).
UniProt Accession Number of Target Protein: P01317
Alternative Name(s) of Target: INS; proinsulin
Immunogen: The original antibody was generated by immunizing female BALB/c mice with calf insulin in Freund's complete adjuvant.
Specificity: This antibody binds an epitope on the B chain of calf, human and rodent insulin. Insulin is a peptide hormone produced by the beta cells of the pancreatic islets. It reduces blood glucose concentration by promoting its absorption from the blood into liver, fat and skeletal muscle cells. Decreased or absent insulin activity results in diabetes mellitus, a condition of high blood sugar level (hyperglycemia).
Application Notes: This antibody can be used to detect insulin in a sample using ELISA and radioimmunoassay (PMID: 2109009; 6193965). This antibody was used in the flow cytometric analysis of insulin occupied B cell receptors in VH125SD B6 mice (PMID: 26109644).
Antibody first published in:
Schroer et al. Mapping epitopes on the insulin molecule using monoclonal antibodies. Eur J Immunol. 1983 Sep;13(9):693-700. PMID:6193965
Note on publication:
Describes the generation of 18 different antibodies to map the antigenic determinants on the human insulin.
Western blot using anti-Insulin antibody Ab 123 (mAb3, CC9C10) (Ab03132). Human pancreas tissue lysate (35µg protein in RIPA buffer) was resolved via SDS-PAGE, and the subsequent blot was probed with the chimeric rabbit version of Ab 123 (mAb3, CC9C10) (Ab03132-23.0) at 0.1µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.