United Kingdom (UK)
Recombinant monoclonal antibody to Integrase. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody scFvs 135.
UniProt Accession Number of Target Protein: P04585
Alternative Name(s) of Target: IN protein; Gag-Pol polyprotein
Immunogen: The original antibody was generated by immunizing rabbits from the New Zealand white strain with HIV-1 IN protein developing a combinatorial single-chain variable fragment (scFv) library against IN. The antibody was isolated by panning against immobilized HIV-1 IN protein.
Specificity: The antibody is specific for the catalytic and C-terminal domains of IN protein. The antibody does not bind to HIV-1 PR protein. This enzyme catalyses the integration of the HIV genome into the chromosome of the host cell. The HIV-1 IN consists of a 288 amino-acid (aa) protein (32 kDa) encoded at the 3′-end of the HIV pol gene and it contains three distinct domains: N-terminal, catalytic core, and the C-terminal.
Application Notes: The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody detected the HIV-1 IN protein by western blot analysis. The scFv fragment inhibited the activity of HIV-1 IN in vitro. HIV-1 replication was inhibited by the scFv localized in cytoplasm and nucleus of HeLa cells. Further, the scFv fragment specifically inhibited HIV-1 DNA integration in Hela-P4 cells. Jurkat cell lines stably expressing scFv intrabody were employed to determine whether the intracellular expression of the scFv fragment was able to prevent HIV-1 replication. Jurkat–scFv fragment cell lines showed approximately 60%–80% inhibition of HIV-1 p24 antigen production compared with the parental Jurkat cells (da Silva et al., 2012; PMID: 23586912).
Antibody first published in:
da Silva et al. Recombinant rabbit single-chain antibodies bind to the catalytic and C-terminal domains of HIV-1 integrase protein and strongly inhibit HIV-1 replication Biotechnol Appl Biochem. Sep-Oct 2012;59(5):353-66. PMID:23586912
Note on publication:
The original paper describes the generation and characterization of the antibody.