Anti-Jo-1 [5B5]

Name: Anti-Jo-1 [5B5]
SKU: Ab03409
Availability: InStock

Description:

Recombinant monoclonal antibody to Jo-1. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the chicken B-cell clone 5B5.

Clone Name:

5B5

Published Application(s):

WB, ELISA

Published Species Reactivity:

Human

Products based on this clone:

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Ab03409-23.0 Anti-Jo-1 [5B5]
Human
Rabbit IgG
Purified
In Stock

Ab03409-30.11 Anti-Jo-1 [5B5]
Human
scFv fragment (His)
ScFv
Purified
Ships in 5-6 weeks

UniProt Accession Number of Target Protein: P12081

Alternative Name(s) of Target: histidyl tRNA synthetase; Histidine--tRNA ligase, cytoplasmic; HisRS
Immunogen: The original antibody was generated by immunizing a barnevelder chicken with an antibody antigen cocktail comprising recombinant purified human antigens annexin XI, CENP-B, Topo, Jo-1, Ro60, Rpp38 and U1A in complete Freund's adjuvant. B cells that were harvested from the bone marrow and peripheral blood lymphocytes of immunized chickens was used to generate recombinant scFv phage display libraries. The specific clone was identified by panning the library against specific antigen.

Specificity: The antibody is specific for Jo-1. Histidyl-tRNA synthetase is a member of the aminoacyl-tRNA synthetase family, it catalyzes the ATP-dependent ligation of histidine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (His-AMP). Jo-1 represents a key autoantibody target in the anti-synthetase syndrome that is marked by myositis as well as extra-muscular organ complications including interstitial lung disease (ILD).

Application Notes: The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody detected Jo-1 by western blot analysis (Hof et al., 2008; PMID: 18062792).

Antibody first published in: Hof et al. Multiple-antigen immunization of chickens facilitates the generation of recombinant antibodies to autoantigens Clin Exp Immunol. 2008 Feb;151(2):367-77. PMID:18062792
Note on publication: Describes the generation of chicked antibodies against various human autoantigens.

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