United Kingdom (UK)
Recombinant monoclonal antibody to KRT19. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody KE9.
UniProt Accession Number of Target Protein: P08727
Alternative Name(s) of Target: Keratin-19; K19; CK-19; Cytokeratin-19; Keratin type I cytoskeletal 19
Immunogen: This antibody was raised panning against a library created by immunizing llamas with either breast cancer biopsy lysates.
Specificity: This antibody is specific for human KRT19. KRT19 is involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Application Notes: To help identify the target of this antibody immunoprecipitation was preformed on T47D cells using the VHH version of this antibody bound to streptavidin beads. Further the ability of this antibody to be used for breast cancer research was assayed using immunohistochemistry. In short, breast cancer tissue was stained using the VHH version of this antibody. Finally to characterize this antibody. An ELISA was preformed using the VHH version of this antibody on KRT19 (Even-Desrumeaux et al, 2012; pmid22772166).
Antibody first published in:
Desrumeaux et al. Single-domain antibodies: a versatile and rich source of binders for breast cancer diagnostic approaches. Mol Biosyst. 2012 Sep;8(9):2385-94. PMID:22772166
Note on publication:
phage display libraries from animals immunized with breast cancer biopsies were generated. These libraries were used to isolate sdAbs against known and relevant antigens such as HER2, or several cancer-specific sdAbs against unknown targets.