Anti-LDL Receptor [C7]

Name: Anti-LDL Receptor [C7]
SKU: Ab03080
Availability: InStock

Description:

Recombinant monoclonal antibody to LDL Receptor. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma C7.

Clone Name:

Published Application(s):

inhibition, IP, WB, FC, IF

Published Species Reactivity:

Bovine, Human

Products based on this clone:

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Ab03080-23.0 Anti-LDL Receptor [C7]
Bovine, Human
Rabbit IgG
Purified
In Stock

Ab03080-3.0 Anti-LDL Receptor [C7]
Bovine, Human
Mouse IgG2b
-
In Stock

Ab03080-10.0 Anti-LDL Receptor [C7]
Bovine, Human
Human IgG1
Purified
Ships in 4-5 weeks

Ab03080-10.3 Anti-LDL Receptor [C7]
Bovine, Human
Human IgG1
Fc Silent™
Purified
Ships in 4-5 weeks

Ab03080-2.0 Anti-LDL Receptor [C7]
Bovine, Human
Mouse IgG2a
Purified
In Stock

UniProt Accession Number of Target Protein: P01130

Alternative Name(s) of Target: LDLR; Low-density lipoprotein receptor; IgG-C7
Immunogen: The original antibody was produced by immunization of BALB/C mice with the extra cellular domain of the bovine LDL-R.

Specificity: The antibody recognizes the ligand binding domain. It binds to the bovine LDL-R, the human LDL-R but it does not cross react with the LDL-Rs of rat, mouse, Chinese hamster, rabbit and dog. The LDL receptor is a cell surface protein that binds LDL, the major cholesterol-transport protein in plasma, and mediates its endocytosis.

Application Notes: The antibody (IgG2b) was employed for the staining of the LDL receptor in normal fibroblast. The antibody competed with LDL for binding to the LDL receptor of intact fibroblast; complete inhibition was achieved at 37 C but not at 4 C (Beisiegel et al., 1981; PMID: 6271765). The antibody detected LDL receptor from human fibroblasts and bovine adrenal cortex by western blot analysis (Beisiegel et al., 1982; PMID: 6290495). The LDL receptor was immunoprecipitated using this antibody (Davis et al., 1987; PMID: 3494949). This antibody was used for detection of LDL receptor expressed on Chang and CHO cells by flow cytometry (Holst et al., 2001; PMID: 11781697) (Ranheim et al., 2006; PMID: 16740646). Immunofluorescence was performed on liver Chang cells using this antibody, showing that the WT protein was localised on the cell surface whereas the two mutant proteins were localised intracellularly (Holst et al., 2001; PMID: 11781697). The antibody was used to inhibit the binding of LDL to its receptor LDLR in brain capillary endothelial cells. Coincubations of LDL with increasing concentrations of the antibody decreased the rate of passage of the LDL through the monolayer; LDL transcytosis was totally abolished at the concentration of 1 mg/ml of antibody. This confirmed that the LDL receptor is involved receptor-mediated transcytosis (Dehouck et al., 1997; PMID: 9265653).

Antibody first published in: Beisiegel et al. Monoclonal Antibodies to the Low Density Lipoprotein Receptor as Probes for Study of Receptor-mediated Endocytosis and the Genetics of Familial Hypercholesterolemia J Biol Chem. 1981 Nov 25;256(22):11923-31. PMID:6271765
Note on publication: The paper describes the generation and characterization of the antibody. The antibody was used as a probe for the study of the cellular and genetic factors involved in receptor-mediated endocytosis.

Immunofluorescence staining of HepG2 cells using Ab03080-3.0. Click on image for more details.

Immunofluorescence staining of HepG2 cells with anti-LDL Receptor antibody C7 (Ab03080). Immunofluorescence analysis of paraformaldehyde-fixed HepG2 cells on Shi-fix™ coverslips stained with the mouse IgG2b version of C7 (Ab03080-3.0) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic and membrane staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, Ab03080-3.0, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-3.0) followed by staining with Alexa Fluor® 488 secondary antibody.

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