United Kingdom (UK)
Recombinant monoclonal antibody to Lamin A. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 133A2.
UniProt Accession Number of Target Protein: P02545
Alternative Name(s) of Target: Prelamin-A/C; Lamin-A/C
Immunogen: The original antibody was generated by immunizing a BALB/c mouse with partially purified recombinant human lamin A.
Specificity: The antibody recognizes an epitope located between residues 598-611 of lamin A.
Application Notes: The antibody detected lamin A by western blot analysis (Hozák et al., 1995; PMID: 7769007 and Broers et al., 1997; PMID: 9243284). The original format of the antibody strongly stained all tubular epithelium cells of kidney by immunohistochemistry (Broers et al., 1997; PMID: 9243284). The original antibody was used for staining of Sertoli cells, Leydig cells and Peritubular cells in normal testis and parenchyma adjacent to TGCT by immunohistochemistry (Machiels et al., 1997; PMID: 9274531). This antibody was used for detection of lamin A expressed on CHO-K1 cells by flow cytometry (Broers et al., 1999; PMID: 10504295). The original antibody was used to stain lamin A on late apoptotic cell (Broers et al., 2002; PMID: 12553668). The antibody detected laimin A in fibroblast cells by immunofluorescence analysis (van Tienen et al., 2019; PMID: 30420677).
Antibody first published in:
Hozák et al. Lamin proteins form an internal nucleoskeleton as well as a peripheral lamina in human cells J Cell Sci. 1995 Feb;108 ( Pt 2):635-44. PMID:7769007
Note on publication:
The original paper describes the generation and characterization of the antibody.
Immunofluorescence staining of HeLa cells with anti-Lamin A antibody 133A2 (Ab03358). Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton, on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 133A2 (Ab03358-23.0) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, Ab03358-23.0, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.
Western blot using anti-Lamin A antibody 133A2 (Ab03358). HepG2 cells nuclear lysate (35µg protein in RIPA buffer) was resolved via SDS-PAGE, and the subsequent blot was probed with the chimeric rabbit version of 133A2 (Ab03358-23.0) at 0.3µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.