United Kingdom (UK)
Recombinant monoclonal antibody to Lipid A. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma S1-15.
Alternative Name(s) of Target: lipA
Immunogen: The original antibody was generated by immunizing BALB/c mice with lipA.
Specificity: The antibody recognizes the carbohydrate backbone of lipid A. The antibody binds to the 4'-monophosphoryl GlcN disaccharide. The antibody does not cross react with lipopolysaccharides (LPS). The antibody cross reacts with ssDNA. Lipid A is the lipid component of LPS contained in gram-negative bacteria. Lipid A is the endotoxic principle of LPS.
Application Notes: In passive immunohemolysis, the antibody reacted with bisphosphoryl compounds as well as with the 4'-monophosphoryl partial structures (Kuhn et al., 1992; PMID 1375194). The specificity of the antibody was determined by ELISA analysis. The crystal structures of the Fab fragment in complex with lipid A carbohydrate backbone and in the unliganded form were determined by X-ray diffraction, showing the binding mechanism of the antibody is a lock and key type. Further, the structure of the Fab fragment in complex with single-stranded DNA fragment 5(dT) oligonucleotide was determined. The antibody bound two separate oligonucleotides by their terminal 5- and 3-phosphate groups (Haji-Ghassemi et al., 2015; PMID: 26085093). Solid-phase enzyme immunoassay shown the antibody binds better to the glycoconjugate at low antigen concentrations and better to the lipid A when high concentrations of antigen were used (Brade et al., 1993). The antibody was tested against the synthetic lipid A precursor Ia and compound B1047, a precursor Ia, by enzyme immunoassay. The antibody bound to precursor Ia, but not to compound B 1047 or Escherichia coli Re LPS (Brade et al., 1997; PMID: 9284181).
Antibody first published in:
Kuhn et al. Characterization of the epitope specificity of murine monoclonal antibodies directed against lipid A. Infect Immun. 1992 Jun;60(6):2201-10. PMID:1375194
Note on publication:
The paper describes the generation and characterization of the antibody.