Anti-Lysozyme [D1.3]

Name: Anti-Lysozyme [D1.3]
SKU: Ab02065
Availability: InStock

Description:

Recombinant monoclonal antibody to Lysozyme. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma D1.3.

Clone Name:

D1.3

Published Application(s):

ELISA, FC

Published Species Reactivity:

Chicken

Products based on this clone:

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Ab02065-1.1 Anti-Lysozyme [D1.3]
Chicken
Mouse IgG1
Purified
In Stock

Ab02065-23.0 Anti-Lysozyme [D1.3]
Chicken
Rabbit IgG
Purified
In Stock

UniProt Accession Number of Target Protein: P00698

Alternative Name(s) of Target: HEL; Hen Egg white Lysozyme; Lysozyme C; 1,4-beta-N-acetylmuramidase C; Allergen Gal d IV; Allergen: Gal d 4
Immunogen: The original antibody was generated by immunizing BALB/c mice with hen egg white lysozyme.

Specificity: This antibody specifically binds hen egg-white lysozyme.

Application Notes: Lysozyme is an enzyme that degrades polysacchardies of various types of bacterial cell walls. It is observed that lysozyme bound D1.3 still retains its enzymatic activity. This is attributed to the fact that D1.3 binds lysozyme at a site remote from the catalytic site and that there is no significant conformational change caused by the binding (PMID: 3614217). The three dimension structure of a complex between the Fab from antibody D1.3 and its antigen, HEL, has been determined at 6 and at 2.8 a resolution (PMID: 2426778, 3965976). Sixteen residues of lysozyme make contact with 17 residues of the antibody. The antigenic determinant is topographic including lysozyme residues 18, 19, 21–25, 27, 116–121, 124, and 129. All six complementarity determining regions of the antibody contact the antigen, and in addition, two residues from “framework” segments of V H and V L (Amit et al., 1986; PMID: 2441250). D1.3 binds native lysozyme in an ELISA (PMID: 2448386). Humanized version of this antibody is also available (PMID: 1560463, 2451287). Flow cytometry was used to find the fine difference in affinity of D1.3 (scFv) and mutant M3 clone expressed in a yeast surface display (PMID:10662486).

Antibody first published in: Mariuzza et al. Preliminary Crystallographic Study of the Complex Between the Fab Fragment of a Monoclonal Anti-Lysozyme Antibody and Its Antigen. J Mol Biol, (1983) 15;170(4):1055-8. PMID:6644814
Note on publication: Describes the crystallographic study of the monoclonal antibody bound to lysozyme.

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