United Kingdom (UK)
Recombinant monoclonal antibody to Lysozyme. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody cAbHul6.
UniProt Accession Number of Target Protein: P61626
Alternative Name(s) of Target: 1,4-beta-N-acetylmuramidase C; cAb-Hul6; NbHuL6
Immunogen: Phage libraries were generated using the mRNA extracted from the lymphocytes of immunized dromedaries. The VHH antibody was isolated by biopanning from the libraries.
Specificity: The antibody is specificity for human lysozyme. It does not cross react with hen lysozyme.
Application Notes: The binding affinity of the VHH fragment for human lysozyme was measured by surface plasmon resonance (Kd = 0.7 nM) (Dumoulin et al., 2002; PMID: 11847273). The antibody stabilized an unstable human lysozyme mutant that forms fibrils and amyloids.The crystal structure of the original version of the antibody in complex with human lysozyme was determined (Dumoulin et al., 2003; PMID: 12917687). The original antibody was humanized and the binding affinity for human lysozyme was measured by surface plasmon resonance (Kd = 2.36 nM). The crystal structure of the humanized version of the antibody in complex with human lysozyme was determined (Vincke et al., 2009; PMID: 19010777).
Antibody first published in:
Dumoulin et al. Single-domain antibody fragments with high conformational stability. Protein Sci. 2002 Mar;11(3):500-15. doi: 10.1110/ps.34602. PMID:11847273
Note on publication:
The original paper describes the generation and characterization of the antibody