United Kingdom (UK)
Recombinant monoclonal antibody to MCSP. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma LC007 (M4-3-ML2).
UniProt Accession Number of Target Protein: Q6UVK1
Alternative Name(s) of Target: CSPG4; Chondroitin sulfate proteoglycan 4; Melanoma-associated Chondroitin Sulfate Proteoglycan; Melanoma chondroitin sulfate proteoglycan; Chondroitin sulfate proteoglycan NG2
Immunogen: The original antibody was generated by immunizing balb/c mice with a synthetic linear peptide corresponding to aa 2177-2221 of the human MCSP, derived from the membrane proximal D3 domain.
Specificity: This antibody recognizes a membrane-proximal epitope on the D3 domain of human MCSP. It also cross reacts with MCSP from cynomolgus monkey.
Application Notes: The binding characterization of the original mouse version of this antibody was done using ELISA. This antibody can also binds to native form of MCSP expressed on Colo38 cells in a flow cytometric analysis. This antibody can also be used for immunofluorescence staining of MCSP in Colo38 cells. The original mouse version can also detect MCSP fragments in a western blot (Freytag et al., US9309306). The original mouse version of this antibody LC007 is capable of inducing antibody dependent cellular cytotoxicity. This antibody can bind the native epitope on MCSP+ melanoma cells, but there is no induction of internalization. This antibody can be used for immunohistochemical staining of MCSP+ cells in formalin fixed paraffin embedded tissue. It was reported that glycoengineering of LC007 antibody using GlycoMab technology resulted in increased binding affinity for hFcgRIIIa and enhanced ADCC potency and absolute killing of melanoma cell lines. (Mössner et al, Abstract LB-236- AACR 103rd Annual Meeting 2012, Mar 31‐Apr 4, 2012; Chicago, IL). The CD16 158 V variant of CD16-CAR T cells showed increased cytotoxic activity against all the tested cancer cells in the presence of the original mouse version of this antibody (PMID: 30429531).
Antibody first published in: Anti-MCSP antibodies. US9309306 PMID: