United Kingdom (UK)
Recombinant monoclonal antibody to MESD. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma N88/12.1.
UniProt Accession Number of Target Protein: Q9ERE7
Alternative Name(s) of Target: N88/12.1R; LRP chaperone MESD; Mesoderm development candidate 2; Mesoderm development protein
Immunogen: This antibody was raised by immunising BALB/c mice with a fusion protein of amino acids 30-95 and 118-180 (lacking VSD domain) of mouse MESD.
Specificity: This antibody is specific for amino acids 30-95 and 118-180 (lacking VSD domain) of mouse MESD. MESD is a chaperone specifically assisting the folding of beta-propeller/EGF modules within the family of low-density lipoprotein receptors (LDLRs).
Application Notes: This antibody is recommended for detection and analysis of MESD by immunohistochemistry, western blot or immunoprecipitation. For instance, the mouse version of this antibody was used to detect MESD by immunohistochemistry in adult rat hippocampus.
Antibody first published in:
Andrews et al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain eLife. 2019; 8: e43322. PMID:30667360
Note on publication:
Describes the generation of a library of recombinant monoclonal antibodies (R-mAbs) from a pool of mAb-producing hybridomas for neuroscience research.