United Kingdom (UK)
Recombinant monoclonal antibody to MMACHC. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma N230/21.
UniProt Accession Number of Target Protein: Q9Y4U1
Alternative Name(s) of Target: N230/21R; Cyanocobalamin reductase / alkylcobalamin dealkylase; Alkylcobalamin:glutathione S-alkyltransferase; CblC; Cyanocobalamin reductase (cyanide-eliminating); Methylmalonic aciduria and homocystinuria type C protein
Immunogen: This antibody was raised by immunising BALB/c mice with a fusion protein amino acids 1-282 (full-length) of human MMACHC.
Specificity: This antibody is specific for the MMACHC protein. MMACHC is a cobalamin (vitamin B12) cytosolic chaperone that catalyzes the reductive decyanation of cyanocob(III)alamin (cyanocobalamin, CNCbl) to yield cob(II)alamin and cyanide, using FAD or FMN as cofactors and NADPH as cosubstrate.
Application Notes: This antibody was used to preform a western blot on lysated mouse embryos (Moreno-Garcia et al, 2014; PMID:24889031).
Antibody first published in:
Moreno-Garcia et al The Mmachc gene is required for pre-implantation embryogenesis in the mouse Mol Genet Metab. 2014 Jul;112(3):198-204. PMID:24889031
Note on publication:
This article describes the research on the inheritance of the MMACHC gene in mice