United Kingdom (UK)
Recombinant monoclonal antibody to MSH6. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma ABM58060-20A2-PU.
UniProt Accession Number of Target Protein: P52701
Alternative Name(s) of Target: hMSH6; p160; GTBP; GTMBP; DNA mismatch repair protein Msh6; G/T mismatch-binding protein; MutS protein homolog 6; MutS-alpha 160 kDa subunitC; GMCC NO.17492; 44
Immunogen: The original antibody was generated by immunizing BALB/c mice with recombinant human MSH6 protein.
Specificity: This antibody binds human DNA mismatch repair protein MSH6. MSH6 forms a heterodimer with MSH2 to make the human MutSα mismatch repair complex and initiates DNA repair. The DNA mismatch repair system (MMR) is composed of enzyme molecules that specifically repair DNA base mismatches. Defects in expression of MSH6 are often associated with hereditary non-polypatopathic colorectal cancer (HNPCC).
Application Notes: The binding specificity of this antibody for human MSH6 was determined using ELISA. This antibody was capable of detecting recombinant MSH6 in a western blot. This antibody was also used in the immunohistochemical analysis of colon cancer, bladder cancer and testicular tissues. This antibody shows similar staining of MSH6 protein compared to the commercially available antibody 44 (CN111454365).
Antibody first published in: PMID:
Flow cytometry using anti-MSH6 antibody ABM58060-20A2-PU (Ab03192). Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with the anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of ABM58060-20A2-PU (Ab03192-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000, and the cells were analyzed using a FACSCanto flow-cytometer.
Immunofluorescence staining of HeLa cells with anti-MSH6 antibody ABM58060-20A2-PU (Ab03192). Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton, on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of ABM58060-20A2-PU (Ab03192-23.0) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, Ab03192-23.0, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.