United Kingdom (UK)
Recombinant monoclonal antibody to Methotrexate. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the llama peripheral blood lymphocytes R3.04.
Alternative Name(s) of Target: MTX; amethopterin; 4-amino-N10-methylpteroylglutamic acid; 4-amino-10-methylfolic acid; CHEBI:44185; CAS number: 59-05-2
Immunogen: The original antibody was generated by immunizing a female llama with methotrexate–BIP conjugate.
Specificity: This antibody binds methotrexate, formerly known as amethopterin. It is a chemotherapy agent and immune-system suppressant. It is often used in treatment of cancer, autoimmune diseases, ectopic pregnancy and for medical abortions.
Application Notes: The binding reactivity of this single chain antibody for methotrexate-BSA was done using direct and competitive ELISA, with no cross reactivity with only BSA. The binding affinity of the original VHH version of this antibody as determined by BIAcore analysis was reported to be Kd=29 nM (PMID: 17011035).
Antibody first published in:
Rueda et al. Generation of llama single-domain antibodies against methotrexate, a prototypical hapten. Mol Immunol. 2007 Mar;44(7):1680-90. PMID:17011035
Note on publication:
Describes the generation and characterization of this antibody.