United Kingdom (UK)
Recombinant monoclonal antibody to Mitofusin-2. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma N153/5.1.
UniProt Accession Number of Target Protein: Q80U63
Alternative Name(s) of Target: N153/5.1R; N153/5; Hypertension-related protein 1; Mitochondrial assembly regulatory factor; HSG protein; Transmembrane GTPase MFN2
Immunogen: This antibody was raised by immunising BALB/c mice with a fusion protein corresponding to Mouse Mitofusin 2 aa 370-600.
Specificity: This antibody is specific for the mitofusion-2 protein and shows no cross reactivity with mitofusion-1. Mitofusion-2 is a mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion
Application Notes: This antibody is recommended for the detection and analysis of mitofusin-2 by western blot, immunofluorescence and immunocytochemistry. For instance, the mouse version of this antibody was used to detect Mitofusin-2 by western blot in extract of crude cardiac mitochondrial preps from WT, Mfn1 KO or Mfn2 KO mice. It was also used for immunohistochemistry in which Mitofusin-2 was detected in adult rat hippocampus.
Antibody first published in:
Andrews et al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain eLife. 2019; 8: e43322. PMID:30667360
Note on publication:
This article describes the generation of a library of recombinant monoclonal antibodies (R-mAbs) from a pool of mAb-producing hybridomas for neuroscience research.