United Kingdom (UK)
Recombinant monoclonal antibody to Morphine. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 9B1.
Alternative Name(s) of Target: Morphium; 6-MAM; 6-monoacetylmorphine; heroin; hydrocodone; opoids; opium; ChEBI: 17303
Immunogen: The original antibody was generated by immunizing mouse with 3-carboxymethyl-morphine conjugated to bovine serum albumin.
Specificity: This antibody binds morphine and cross reacts with multiple addictive opioids including 6-MAM, morphine, heroin, and hydrocodone, shows weak binding for meperidine, oxycodone and fentanyl without significant binding to currently available OUD treatment agents naloxone, naltrexone, and buprenorphine. Morphine is a strong opiate that is found naturally in opium, a dark brown resin in poppies (Papaver somniferum). It is considered the classic opioid analgesic, most commonly used in pain management, morphine provides major relief to patients afflicted with pain. It is a non-synthetic narcotic with a high potential for abuse.
Application Notes: The original IgG antibody binds morphine with a binding affinity of KD= 33.7nM, and also binds 6-MAM (Kd = 4.8 nM), heroin (Kd = 1.3 nM) and hydrocodone (Kd= 8.2 nM) (PMID: 33356138). The binding ability of this antibody to a variety of drugs was determined using ELISA (PMID: 33356138).
Antibody first published in:
Pozharski et al. Anchoring a cationic ligand: the structure of the Fab fragment of the anti-morphine antibody 9B1 and its complex with morphine. J Mol Biol. 2004 Mar 26;337(3):691-7. PMID:15019787
Note on publication:
Describes the crystal structure of this antibody in combination with morphine.