United Kingdom (UK)
Recombinant monoclonal antibody to Myelin basic protein. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 845D3.
UniProt Accession Number of Target Protein: P02686
Alternative Name(s) of Target: MBP; Myelin A1 protein; Myelin membrane encephalitogenic protein; 84-5D3
Immunogen: The original antibody was generated by hyperimmunization of BALB/c mice with human MBP peptide comprising amino acids from 80-89.
Specificity: This antibody binds human myelin basic protein (MBP), amino acid residues 80-89 (QDENPVVHF). This antibody also cross reacts with bovine MBP comprising amino acids 78-88. The MBP isoforms 4 and 14 along with PLP are most abundant protein components of the myelin membrane in the CNS. The smaller isoforms are believed to have an important role in remyelination of denuded axons in multiple sclerosis.
Application Notes: The epitope mapping of this antibody was done using solid phase radioimmunoassay (PMID: 2419434). This antibody can be used in the dection of MBP in a sample using ELISA (PMID: 2466871).
Antibody first published in:
Price et al. Multiple epitopes in a dodecapeptide of myelin basic protein determined by monoclonal antibodies. J Immunol. 1986 Apr 1;136(7):2426-31. PMID:2419434
Note on publication:
Describes the generation of 4 antibodies against different epitopes of myelin basic protein and their epitope characterization using solid phase radio immunoassay.