United Kingdom (UK)
Recombinant monoclonal antibody to Myelin basic protein. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the human B cell clone B5V6.
UniProt Accession Number of Target Protein: P02687
Alternative Name(s) of Target: MBP; Myelin A1 protein; Myelin membrane encephalitogenic protein
Immunogen: The original antibody was isolated from a phage display library of single-chain variable fragments (scFvs), which was constructed from blood lymphocytes of patienst with MS as a potential source of representative MS autoantibodies.
Specificity: This antibody binds bovine myelin binding protein (MBP) both in its native and denatured form in the region between amino acids 130–156. The MBP isoforms 4 and 14 along with PLP are most abundant protein components of the myelin membrane in the CNS. The smaller isoforms are believed to have an important role in remyelination of denuded axons in multiple sclerosis.
Application Notes: The scFv version of this antibody binds bovine myelin basic protein with a binding affinity of 12 nM in an ELISA (PMID: 21859892).
Antibody first published in:
Gabibov et al. Combinatorial antibody library from multiple sclerosis patients reveals antibodies that cross-react with myelin basic protein and EBV antigen. FASEB J. 2011 Dec;25(12):4211-21. PMID:21859892
Note on publication:
Describes the isolation of antibodies against myelin basic protein and EBV antigens from a combinatorial library generated from blood lymphocytes of patienst with multiple sclerosis.