United Kingdom (UK)
Recombinant monoclonal antibody to NK1.1. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma PK-136.
UniProt Accession Number of Target Protein: P27814
Alternative Name(s) of Target: CD161c; Killer cell lectin-like receptor subfamily B member 1C; CD161 antigen-like family member C; Lymphocyte antigen 55c; Ly-55c; NK1.1; NKR-P1.9; NKR-P1C; Natural killer cell surface protein P1-40; NKR-P1 40; PK136
Immunogen: The original antibody was generated by immunizing (C3H X BALB)F1 mice with spleen cells enriched for NK-1 cells and bone marrow cells from CE mice.
Specificity: The antibody reacts with murine NK1.1. NK1.1 is a type II integral membrane glycoprotein with a C-type lectin domain. NK1.1 plays a stimulatory role on natural killer (NK) cells cytotoxicity. Its activation by cross-linking of the receptor induces Ca2+ mobilization and interferon-gamma production.
Application Notes: The antibody was employed for FACS analysis of CD4+8- thymocytes (Arase et al., 1993; PMID: 8419184). The antibody was used for detection of NK1.1 of spleen cells by flow cytometry (Christianson et al., 1996; PMID: 8806787). The antibody was further used for the detection of NK1.1 by flow cytometry analysis (Arase et al., 1992; PMID: 1378629 and Lee et al., 2018; PMID: 30333822 and Coquet et al., 2008; PMID: 18685112). The antibody was employed for in vivo studies, the efficacy of the antibody in depleting the animals of ΝΚ1.1+ or NK T cells was ascertained by flow cytometric analysis. The reduction was greater than 90% after 1 week of treatment (Nomizo et al., 2005; PMID: 16162272). Finally, the antibody was used for the depletion of NK1.1+ cells in euthymic and thymectomized young C57Bl/6 mice infected with T. cruzi. The depletion of NK1.1 cells caused an increase in the parasitemia in young euthymic mice, but produced no such effects in thymectomized young mice infected with T. cruzi (Tulahuen strain) (Cardillo et al., 2002; PMID: 11841697). The original hybridoma expressed two light chain transcripts, which are both present in the final antibody protein, with only one showing full functionality, with the other light chain potentially mediating off-target binding. This recombinant antibody only features the fully functional light chain, improving antibody performance and on-target specificity.
Antibody first published in:
Koo et al. Establishment of monoclonal anti-Nk-1.1 antibody Hybridoma
. Fall 1984;3(3):301-3. PMID:6500587
Note on publication:
The original paper describes the generation and characterization of the original antibody
ELISA using PK-136 (Ab02764) and NK1.1 mIgG1 Fc fusion (Pr00588). Binding curves of the rabbit IgG chimeric version of the Anti-NK1.1 antibody PK-136 (Ab02764-23.0; blue line) and isotype control (Ab00102-23.0, anti-fluorescein; black line) to an ELISA plate coated with NK1.1 mIgG1 Fc fusion (Pr00588, Absolute Antibody) at a concentration of 2.5 ug/ml. A 3-fold serial dilution from 3000 to 0.0169 ng/ml was performed using Ab02764 antibody. For signal detection, a 1:4000 dilution of a HRP-conjugated anti-rabbit IgG antibody was used.