United Kingdom (UK)
Recombinant monoclonal antibody to Osteocalcin. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma KTM219.
UniProt Accession Number of Target Protein: P02818
Alternative Name(s) of Target: BGP; BGLAP; Bone Gla protein; Gamma-carboxyglutamic acid-containing protein
Immunogen: The original antibody was generated by repeatedly immunizing mice with C-terminal hBGP peptide (NH2-FQEAYRRFYGPVCOOH) conjugated with keyhole limpet hemocyanin.
Specificity: This antibody bind the C-terminal end of the human osteocalcin.
Application Notes: This antibody is recommended for detection of low moecular weight peptides by using open sandwich ELISA (OS-ELISA). This antibody detects C-terminal of human osteocalcin in an ELISA. The cloned VH/VL cDNA fragments of this antibody were successful in detecting C-terminal peptides as short as 6 residues in an OS-ELISA. (PMID: 17636882). The VH-MBP and VL-MBP fusion proteins of this antibody were also used in the detection of small biomarker peptide by a micro OS-ELISA integrated into a microfluidic device (PMID: 20024056). The affinity matured version of this antibody O2AG2 was also made using OS-ELISA. It is also available on request (PMID: 20123883). This antibody was also used in the development of a highly sensitive protein based open sandwich immuno-PCR for detection of small biomarkers (PMID: 24025570)
Antibody first published in:
Lim et al. Noncompetitive detection of low molecular weight peptides by open sandwich immunoassay. Anal Chem. (2007); 79(16):6193-200. PMID:17636882
Note on publication:
Describes the generation and use of this antibody in the detection of low molecular weight peptides using open sandwich ELISA.