United Kingdom (UK)
Recombinant monoclonal antibody to PSK. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 5G5.
Alternative Name(s) of Target: Polysaccharide-K; Krestin; beta-glucan β-1->4 main chain with β-1->3 and β-1->6 side chains
Immunogen: The original antibody was generated by immunizing a mouse with the PSK antigen.
Specificity: The antibody is specific for the PSK protein and doesn't bind to other glucans having a similar structure.
Application Notes: ELISA was performed on PSK using this antibody (WO2012077737) (Hoshi et al, 2011; pmid:21249323). Tumor , liver and spleen tissues were used for immunohistochemistry using this antibody (Hoshi et al, 2011; pmid:21249323). This antibody was used for western blot on PSK protein (Hoshi et al, 2011; pmid:21249323).
Antibody first published in:
Hoshi et al. Anti-protein-bound polysaccharide-K monoclonal antibody binds the active structure and neutralizes direct antitumor action of the compound. Oncol Rep. 2011 Apr;25(4):905-13. PMID:21249323
Note on publication:
we investigated whether PSK reaches tumor and immune tissues with its active structure remaining intact, and the direct action of PSK was evaluated by its antitumor effects against MethA fibrosarcomas implanted in immunodeficient NOD/SCID mice.