United Kingdom (UK)
Recombinant monoclonal antibody to Pancreatic alpha-amylase. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the dromedary peripheral blood lymphocytes AMD9.
UniProt Accession Number of Target Protein: P00690
Alternative Name(s) of Target: PA; AMY2; 1,4-alpha-D-glucan glucanohydrolase
Immunogen: The original antibody was generated by immunizing dromedary with the enzyme and isolating specific binders from the VHH cloned from peripheral blood lymphocytes.
Specificity: This antibody binds the active site crevice of the porcine pancreatic alpha-amylase and is a strong amylase inhibitor.
Application Notes: This antibody can bind porcine pancreatic alpha-amylase in an ELISA and inibits the enzyme with an IC50 = 10nM (PMID: 9649422). This antibody interacts with the amino acid residues in the alpha amylase catalytic site and has a strong inhibitary action on the catalytic activity of the enzyme. The inhibitory interaction of AMD9 involved mainly its complementarity-determining region (CDR) 2 loop, whereas the CDR3 loop was small and certainly did not protrude (PMID: 11960990).
Antibody first published in:
Lauwereys et al. Potent enzyme inhibitors derived from dromedary heavy-chain antibodies. The EMBO Journal (1998), Vol.17 No.13, pp.3512–3520. PMID:9649422
Note on publication:
Describes the generation of this antibody