United Kingdom (UK)
Recombinant monoclonal antibody to Peripherin. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma H184.
UniProt Accession Number of Target Protein: P15331
Alternative Name(s) of Target: PRPH; PRPH1
Immunogen: The original hybridoma was generated by fusing islet-infiltrating B lymphocytes collected from F1(NODxNOR) mouse developing insulitis and the NS-1 myeloma cell line in polyethylene glycol (PEG).
Specificity: This antibody binds mouse peripherin, but also cross reacts with human and rat peripherin. Peripherin is a type III intermediate filament protein expressed mainly in neurons of the peripheral nervous system.
Application Notes: This antibody was used for the immunofluorescence staining of peripherin from cryosections (5 um) of pancreata from NOD.RAG-2/ mice, Sprague Dawley rats, and human cadaveric organ donors. The reactivity of this antibody towards several NOD.RAG-2/ mouse organs was also determined using immunofluorescence staining (PMID: 15616012).
Antibody first published in:
Carrillo et al. Anti-peripherin B lymphocytes are positively selected during diabetogenesis. Mol Immunol. 2008 Jun;45(11):3152-62. PMID:18433871
Note on publication:
The original publication describes the generation of hybridoma cell lines of islet-infiltrating B-cells from mice with type 1 diabetes and diabetes-resistant mice, and the characterization of the tissue specificity of the antibodies produced by these hybridomas.