United Kingdom (UK)
Recombinant monoclonal antibody to Phospho-Tau. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the chicken B cell clone pT231/pS235_1.
UniProt Accession Number of Target Protein: P10636
Alternative Name(s) of Target: MAPT; TAU; PHF-tau; PHF; MAPTL; MTBT1; Tau proteins; Phospho-Tau (pT231/pS235); Microtubule‐associated protein tau; Neurofibrillary tangle protein; Paired helical filament-tau
Specificity: This antibody shows binding affinity towards pT231/pS235 peptide of the human tau protein. The binding affinity and specificity of this antibody relied heavily on the phosphorylation at Thr-231.
Application Notes: ELISA was used for the screening of positive clones that bind phosphopeptides. The binding affinity of the IgG antibody towards pT231/pS235 phosphopeptide was found to be Kd = 0.35 nM using surface plasmon resonance. This antibody can recognize the phospho-tau epitopes in full-length human tau. This antibody was also used for the immunohistochemical analysis of the human brain with Alzheimer's disease (PMID: 23148212).
Antibody first published in:
Shih et al. An ultra-specific avian antibody to phosphorylated tau protein reveals a unique mechanism for phosphoepitope recognition. J Biol Chem. 2012 Dec 28;287(53):44425-34. PMID:23148212
Note on publication:
Describes the generation and characterization of extremely specific recombinant chicken antibodies to three phosphoepitopes on the Alzheimer disease-associated protein tau.