United Kingdom (UK)
Recombinant monoclonal antibody to Plakophilin 1. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 10B2.
UniProt Accession Number of Target Protein: Q13835
Alternative Name(s) of Target: PKP1; PKP-1; Plakophilin-1; Band 6 protein; B6P; PKP 1
Immunogen: This antibody was raised by immunizing mice with the N-terminus of human Plakophilin-1 protein.
Specificity: This antibody recognizes Plakophilin-1. It binds the epitope within the first 27 amino acids from the N-terminal region and it does not cross react with Plakophilin 2 and Plakophilin 3. Plakophilins influence development and participate in linking cadherins to cytoskeletal intermediate filaments. Plakophilin-1 is thought to play a role in junctional plaques and contribute to epidermal morphogenesis.
Application Notes: 10B2 is recommedned for the detection and analysis of Plakophilin-1. In order to identify potential perturbations in cell adherence, this antibody was used to detect plakophilin-1 in the skin tissue of patients with epidermolysis bullosa via immunofluorescence (Pigors et al., 2011; pmid: 21320868). Further, this antibody was demonstrated to have a potential diagnostic role in certain diseases, such as Ectodermal Dysplasia/Skin Fragility Syndrome where it was used to differentiate between a diseased and healthy skin samples (Sprecher et al., 2004; pmid: 15086548). It was also shown that 10B2 antibody can detect plakophilin-1 (PKP1) via Western blot (here: in HaCaT protein lysates) (Bonne et al., 2003; pmid: 12707304).
Antibody first published in:
Bonne et al. Defining desmosomal plakophilin-3 interactions J Cell Biol. 2003 Apr 28;161(2):403-16. doi:10.1083/jcb.200303036. Epub 2003 Apr 21. PMID:12707304
Note on publication:
This article describes the earliest characterization and usage of this anti-plakophilin 1 antibody.