United Kingdom (UK)
Recombinant monoclonal antibody to Polyglutamine. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma MW1.
UniProt Accession Number of Target Protein: P42858
Alternative Name(s) of Target: PolyQ; HTT; htt; Huntington disease protein; HD protein; huntingtin; Glutamine repeats; Atrophin-1
Immunogen: This antibody was raised against a 53 amino acid fragment of atrophin-1 containing poly Q (19) and purified from ascites fluid by protein G affinity chromatography.
Specificity: This antibody binds the extended polyglutamine repeats (GQQQQQQQQQQG) of the mutant huntingtin exon 1. It does not bind the wild type Htt.
Application Notes: This antibody strongly prefers to bind the expanded polyQ repeat forms of the mutant huntingtin and displayed no detectable binding to normal huntingtin in Western blot assay. These results suggested that the polyQ domain can assume different conformations that are distinquishable by mAbs. Immunohistochemistry with wild type (WT) and mutant Htt transgenic mouse (R6) brains shows that different antibodies have different staining patterns. MW1 shows punctate cytoplasmic and neuropil staining in immunohistochemical staining (PMID: 11719267). When MW1 scFv is co-transfected in 293 cells with htt exon 1 containing an expanded polyQ domain, it stimulate htt aggregation and apoptosis (PMID: 11792860). The crystal structure of polyQ bound to MW1, reveals that polyQ adopts an extended, coil-like structure. Consistent with the linear lattice model, multimeric MW1 Fvs bind more tightly to longer than to shorter polyQ tracts and, compared with monomeric Fv, bind expanded polyQ repeats with higher apparent affinities (PMID: 17450152).
Antibody first published in:
Ko et al. New anti-huntingtin monoclonal antibodies: implications for huntingtin conformation and its binding proteins. Brain Research Bulletin (2001); Volume 56; Issues 3–4; Pages 319-329 PMID:11719267
Note on publication:
Describes the generation and characterization of the antibody.