United Kingdom (UK)
Recombinant monoclonal antibody to PorA protein. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma MN14C11.6.
UniProt Accession Number of Target Protein: P57041; E6MXW0
Alternative Name(s) of Target: PIA; Protein IA; Class 1 protein; P1.7; P1.7 serosubtype; Major outer membrane protein P.IA; serogroup A; serogroup B
Immunogen: The original antibody was generated immunizing mice with outer membrane protein preparations from Neisseria meningitidis.
Specificity: This antibody recognizes the P1.7 loop of the PorA protein of Neisseria meningitidis. Epitope mapping revealed that this antibody specifically binds a sequence 'ASGQ'. This antibody can bind both A and B serotype of Neisseria meningitidis. The major outer membrane protein PorA of Neisseria meningitidis is the target for bactericidal serosubtyping antibodies and is currently considered as a potential vaccine candidate against group B meningococcal disease.
Application Notes: This antibody showed both bactericidal and opsonophagocytic for P1.7-expressing meningococci. The lowest concentration resulting in >50% killing of strain 44/76-SL was reported to be 0.5 µg/l. The opsonic activity of this antibody was measured against strain 44/76-SL with human peripheral blood polymorphonuclear leukocytes (PMN) as the effector cells and human serum as the complement source. This antibody demonstrated high opsonophagocytic activity with >80% of PMNs exhibiting measurable RB at a concentration of the MAbs of <10 μg/ml. This antibody was opsonic for the test strain 44/76-SL at a concentration of 0.2 μg/ml (PMID: 10722576). This antibody is not capable of binding meningococcal isolates expressing some variations of the P1.7 family especially P1.7b in a whole cell ELISA (PMID: 10512717). This antibody can be used in the detection of outer membrane protein of N. meningitidis strains like 44/76 using an ELISA (PMID: 12753502). This antibody was also used for the analysis of isogenic target strains derived from H44/76 (B:15:P1.7,16) in a western blot assay (PMID: 8698504).
Antibody first published in:
Abdillahi et al. Definition of meningococcal class 1 OMP subtyping antigens by monoclonal antibodies. FEMS Microbiol Immunol. 1988 Dec;1(3):139-44. PMID:3152545
Note on publication:
The paper defines antigenic determinants on the class 1 outer membrane proteins using antibodies.