United Kingdom (UK)
Recombinant monoclonal antibody to PorA protein. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma MN16C13F4.
UniProt Accession Number of Target Protein: Q9JPT2; Q51220; P0DH58
Alternative Name(s) of Target: PorA; Major outer membrane protein; outer membrane porin protein; PIA; Protein IA; Class 1 protein; serosubtype P1.2; serogroup B
Immunogen: The original antibody was generated using outer membrane protein preparations from Neisseria meningitidis as immunogen.
Specificity: This antibody is specific for the P1.2 subtype antigen (HFVQQTPKSQPTL) of serogroup B of Neisseria meningitidis.
Application Notes: The specific binding reactivity of this antibody to the P1.2 subtype antigen peptide 'HFVQQTPKSQPTL' was checked using ELISA (PMID: 16470851). This antibody at a dilution of 1:20,000 is capable of binding live cells of Neisseria meningitidis serogroup B in a flow cytometric assay (PMID: 11349041). This antibody was also used in the visualization of P1.2 epitope on the surface of the vesicles by using immunogold labelling (PMID: 8873395).
Antibody first published in:
Brooks et al. Sequence variation in class 1 outer membrane protein in Neisseria meningitidis isolated from patients with meningococcal infection and close household contacts. FEMS Microbiol Lett. 1995 May 1;128(2):145-50. PMID:7750732
Note on publication:
This paper analysis the variations in the sequence of class 1 outer membrane protein in Neisseria meningitidis isolated from patients.